Formulation and evaluation of the effectiveness hair tonic containing ethanolic extract of garden balsam leaves (Impatiens balsamina L.) using the Tanaka method
DOI:
https://doi.org/10.29303/aca.v9i2.325Keywords:
Hair tonic, Impatiens, hair growth, tanaka methodAbstract
Hair issues such as hair loss and slow growth require safe and effective treatment products. Garden balsam leaves (Impatiens balsamina L.) contain secondary metabolites specifically flavonoids, alkaloids, saponins, and tannins that have the potential to serve as active ingredients in hair tonic formulations. This study aimed to formulate, evaluate the physical quality of, and test the effectiveness of a hair tonic containing an ethanolic extract of garden balsam leaves on hair growth using the Tanaka method. This was a laboratory-based experimental study utilizing the maceration extraction method with 96% ethanol. Three concentrations were formulated F1 (4%), F2 (6%), and F3 (8%) and compared against a negative control (distilled water) and a positive control (5% minoxidil). Physical quality evaluations included tests for organoleptic properties, homogeneity, pH, and viscosity, while effectiveness was analyzed using the Shapiro-Wilk test, homogeneity test, One-Way ANOVA, and the Least Significant Difference (LSD) test. The results showed an extraction yield of 28.3%, with positive test results for flavonoids, alkaloids, saponins, and tannins. All formulations met physical quality standards and promoted hair growth compared to the negative control. Statistical analysis revealed significant differences in effectiveness among the treatment groups (p<0.05). Formula F3 (8%) produced the highest average hair growth, making it the most effective formulation for stimulating hair growth.
Downloads
References
[1] Bagus, I., Mahardika, P., Luh, N., Diah, P., & Saka, I. (2025). Antioxidant activity and phytochemical profile of water Garden Balsam leavesextract (Impatiens balsamina L.) With a variety of solvents. Journal of Malahayati Pharmacy, 8(2), 249–260.
[2] Collins, E., Rollando, & Monica, E. (2023). Manufacture of Hair Growth Serum Combination of Pecan Oil (Aleurites moluccanus) and Apple Fruit Extract (Pyrus malus L.). Journal of Pharmacy Ma Chung: Science, Technology, and Community Clinical, 1((1)), 32–41.
[3] Eni Tri Mastuti, Wicahyo Septian Maulid, & Wardani Tatiana Siska. (2024). Formulation of Hair Tonic Preparation from a Combination of Celery Leaf Ethanol Extract (Apium graveolens L.) and fragrant pandan leaves (Pandanus amaryllifolius Roxb.) As a hair growth stimulant in rabbits (New Zealend White). Tambusai Health Journal, 5(4), 10871–10872.
[4] Hayati, P. P., Pratama, G., Surilayani, D., & Hasanah, A. N. (2024). Formulation of Hair Tonic Preparation of Seaweed Extract (Hormophysa triquetra) with a Combination of Pecan Extract (Aleurites moluccana) as a Hair Grower. Journal of Halal Agroindustry, 10(1), 45–56. https://doi.org/10.30997/jah.v10i1.12008
[5] Koralina, S., Sunarsih, E. S., & Wulandari, F. (2023). Activity Test of Hair Tonic Preparation Ethanol Extract 70 % Bitter Melons (Momordica charantia L.) Against hair growth in rabbits (Oryctolagus cuniculus). Journal of Pharmacy and Pharmacology, 27(3), 103–109. Https://Doi.Org/10.20956/Mff.V27i3.27548
[6] Mahardika, IBP (2025). Antioxidant Activity and Phytochemical Profile of Water Garden Balsam leavesExtract (Impatiens balsamina L.) With a variety of solvents. JFM (Journal of Malahayati Pharmacy), 8 (2), 249-260.
[7] Muliani, W., Setiawan, F., & Sukmawan, Y. P. (2022). Formulation and Evaluation of Hair Tonic Preparation of Katuk Leaf Ethanol Extract (Sauropus androgynus (L.) Merr.) as Hair Growth in New Zealand White Male Rabbits. In Proceedings of the National Seminar on the Dissemination of Research Results of the S1 Pharmacy Study Program, 2, 101–112.
[8] Pandya, P., & Nallanchakravarthula, S. (2022). Determination of the Quantitative Phytochemical Screening and Antioxidant Activity of Impatiens balsamina L. Leaves and Flower under Heavy Metal Stress (Nickel). Environment and Ecology, 40(1), 146–155.
[9] Puspitasari, A., & Sulistyawati, R. (2024). Formulation and Physical Stability Test of Hair Tonic Preparation of Ethanol Extract of Bitter Melons (Momordica charantia L.). IJMS – Indonesian Journal of Medical Science, 11(2), 109–114.
[10] Rantika, N., Hindun, S., Najihudin, A., & Indra, A. (2023). Formulation of Hair Tonic Preparation Ethanol Extract Moringa Leaf (Moringa leifera Lamk.) and Rambutan Leaf (Nephelium lappaceaum L.) Against hair growth. Pharma Xplore: Journal of Pharmaceutical Science and Sciences, 8(1), 65-76.
[11] Rifaldi, M., Ode, W. L., & Hj.Nurlina. (2025). Formulation of Hair Tonic Preparation with Ethanol Extract from Kepok Banana Stem (Musa paradasiaca L) from Pinrang Regency to Overcome Hair Loss. Al-Ghafiqi Journal of Pharmacy (JUFAL), 1(2), 1–6.
[12] Safitri, M., & Novelni, R. (2024). Eligibility of Hair Tonic Bitter Melons (Momordica charantia L.) For hair loss treatment. Journal of Cosmetology, 14(01), 33–45.
[13] Selviana, A.P., Khoirotunnisa, U., Ulandari, A.S., Rahayu, I.D., & Andrifianie, F. (2024). Effect of Ethanol Concentration and Volume on the Yield of Telang Flower Extract (Clitoria ternatea L.) In the ade maceration extraction method. Journal of Health and Agromedicine, 11(2), 94–100.
[14] Suraiya, C., Utami, W., Tinggi, S., Health, I., & Aceh, A. (2025). Hair Tonic Formulation Based on Tea Leaves (Camellia sinensis) as a Hair Growth Stimulator. Thermometer: Scientific Journal of Health and Medical Sciences, 3(1), 302–309.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 I Gusti Ayu Pitari, Mia Ariasti, Lalu Busyairi Muhsin

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with ACA: Acta Chimica Asiana agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in ACA: Acta Chimica Asiana.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Indonesian Chemical Society, Chapter Nusa Tenggara. Jalan Majapahit 62 Mataram, University of Mataram, 83125, Indonesia