Determination of Total Flavonoid Content from Extract and Fractions of Mangrove Leaves (Rhizopora mucronata)

Rahula Vijja Sammanta; Lina Permatasari; Handa Muliasari; Neneng Rachmalia Izzatul Mukhlishah; Rizqa Fersiyana Deccati

doi:10.29303/aca.v7i2.199

$Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves$

Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves

Authors

Rahula Vijja Sammanta , Lina Permatasari , Handa Muliasari , Neneng Rachmalia Izzatul Mukhlishah , Rizqa Fersiyana Deccati

DOI:

10.29303/aca.v7i2.199

Published:

2024-10-31

Issue:

Vol. 7 No. 2 (2024)

Keywords:

Rhizopora mucronata leaves, total flavonoid content, spectrophotometry UV/Vis, Thin Layer Chromatography, Mangrove

Articles

How to Cite

Sammanta, R. V., Permatasari, L., Muliasari, H., Rachmalia Izzatul Mukhlishah, N., & Fersiyana Deccati, R. (2024). Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves. Acta Chimica Asiana, 7(2), 502–508. https://doi.org/10.29303/aca.v7i2.199

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Abstract

Indonesia, an archipelagic country, includes Lombok Island, known for its extensive mangrove forests, where Rhizopora mucronata is the dominant species. Rhizopora mucronata, from the Rhizoporaceae family, is known for its antibacterial, antifungal, and antioxidant properties due to its secondary metabolites, including flavonoids. However, research on the flavonoid content of its leaves remains unexplored. This study aimed to determine the total flavonoid content in extracts and fractions of R. mucronata leaves using the UV-Vis spectrophotometric method. Leaves were extracted with 96% ethanol using sonication and fractionated with n-hexane, ethyl acetate, and water. The extract and fractions yielded 42.33%, 43.15%, 40.49%, 9.74%, and 32.49%, respectively. TLC tests indicated the presence of flavonoids marked by blue spots. The flavonoid contents in the extract and fractions were 12,980; 14,160; 23,880; and 25,350 mg Quercetin equivalent/gram sample (mg QE/g). The total flavonoid content was analyzed using One-way ANOVA, showing higher levels in the n-hexane and ethyl acetate fractions than in the ethanol extract and water fraction. The high flavonoid content contributes to the biological activities of R. mucronata leaves.

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Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves

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