Gravity column chromatography of n-hexane fraction of Rhizophora mucronata leaves from West Nusa Tenggara and their antioxidant activity

Lina Permatasari; Handa Muliasari; Qori'atul Hafizah; Annisa Rizka Nirmala; Hilkatul Ilmi

doi:10.29303/aca.v8i2.243

$Gravity column chromatography of n-hexane fraction of Rhizophora mucronata leaves from West Nusa Tenggara and their antioxidant activity$

Gravity column chromatography of n-hexane fraction of Rhizophora mucronata leaves from West Nusa Tenggara and their antioxidant activity

Authors

Lina Permatasari , Handa Muliasari , Qori'atul Hafizah , Annisa Rizka Nirmala , Hilkatul Ilmi

DOI:

10.29303/aca.v8i2.243

Published:

2025-11-28

Issue:

Vol. 8 No. 2 (2025)

Keywords:

Rhizpora mucronata leaves fraction, gravity column chromatography, antioxidant activity, ABTS, DPPH

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Permatasari, L., Muliasari, H., Hafizah, Q., Rizka Nirmala, A., & Ilmi, H. (2025). Gravity column chromatography of n-hexane fraction of Rhizophora mucronata leaves from West Nusa Tenggara and their antioxidant activity. Acta Chimica Asiana, 8(2), 752–759. https://doi.org/10.29303/aca.v8i2.243

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Abstract

In the last few decades, many diseases have been caused by Radical Oxidative Stress (ROS) or free radicals. Compounds that act as natural antioxidants have been widely developed to counteract these free radicals. Rhizpora mucronata was a mangrove species reported to possess strong antioxidant activity. However, the research is limited to extracts; fractions with more specific compound components have not been explored. The n-hexane fraction of R. mucronata leaves was reported to have high total flavonoid content (TFC). The TFC has a positive correlation with antioxidant activity. Therefore, this study aimed to simplify the compound components in the n-hexane fraction of R. mucronata leaves using gravity column chromatography (GCG) and determine their antioxidant activity. The n-hexane fraction was fractionated using GCG with increasing solvent polarity (n-hexane, chloroform, ethyl acetate, and methanol). The fractions were tested for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods. The fractionation using GCG resulted in 80 fractions. These fractions were grouped based on their thin-layer chromatography (TLC) profiles, resulting in 12 groups of fractions (A-L). Fractions D, F, G, H, J, K, and L, the fractions with enough yield, determined their antioxidant activity using the DPPH and ABTS methods. Fractions D and J tested by the DPPH method had IC50 values of 28.68 ± 1.58 ppm and 9.59 ± 0.39 ppm, respectively. Meanwhile, fractions D and J tested by the ABTS method had IC50 values of 15.10 ± 1.00 ppm and 3.16 ± 0.55 ppm, respectively. Meanwhile, fractions F, G, H, K, and L exhibit antioxidant activity with IC50 values greater than 100 ppm. Fractions D and J have potent antioxidant activity, and both were tested using DPPH and ABTS methods. Therefore, fractions D and J can be further developed as natural antioxidants.

References

. Phaniendra, A., Jestadi, D. B., & Periyasamy, L. (2015). Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry, 30(1), 11–26

. Zaric, B. L., Macvanin, M. T., & Isenovic, E. R. (2023). Free radicals: Relationship to Human Diseases and Potential Therapeutic applications. The International Journal of Biochemistry & Cell Biology, 154, 106346. https://doi.org/10.1016/j.biocel.2022.106346

. Martemucci, G., Costagliola, C., Mariano, M., D’andrea, L., Napolitano, P., & D’Alessandro, A. G. (2022). Free Radical Properties, Source and Targets, Antioxidant Consumption and Health. Oxygen, 2(2), Article 2. https://doi.org/10.3390/oxygen2020006

. Panova, I. G., & Tatikolov, A. S. (2023). Endogenous and Exogenous Antioxidants as Agents Preventing the Negative Effects of Contrast Media (Contrast-Induced Nephropathy). Pharmaceuticals, 16(8), Article 8. https://doi.org/10.3390/ph16081077.

. Bhuyan, U., & Handique, J. G. (2022). Chapter 6 - Plant polyphenols as potent antioxidants: Highlighting the mechanism of antioxidant activity and synthesis/development of some polyphenol conjugates. In Atta-ur-Rahman (Ed.), Studies in Natural Products Chemistry (Vol. 75, pp. 243–266). Elsevier. https://doi.org/10.1016/B978-0-323-91250-1.00006-9

. Andrés, C. M. C., Pérez de la Lastra, J. M., Juan, C. A., Plou, F. J., & Pérez-Lebeña, E. (2024). Antioxidant Metabolism Pathways in Vitamins, Polyphenols, and Selenium: Parallels and Divergences. International Journal of Molecular Sciences, 25(5), Article 5. https://doi.org/10.3390/ijms25052600.

. Sammanta, R. V., Permatasari, L., Muliasari, H., Mukhlishah, N. R. I., & Deccati, R. F. (2024). Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves | Acta Chimica Asiana. https://aca.unram.ac.id/index.php/ACA/article/view/199

. Rumengan, A. P., Mandiangan, E. S., Tanod, W. A., Paransa, D. S. J., Paruntu, C. P., & Mantiri, D. M. H. (2021). Identification of pigment profiles and antioxidant activity of Rhizophora.

. Permatasari, L., Muliasari, H., & Ilmi, H. (2025). Principal Component Analysis (PCA) of Total Phenolic Content, Antioxidant and Antimalarial Activities of Rhizopora mucronata, Avicennia marina, and Sonneratia alba Leaves from Lombok Island. Research Journal of Pharmacy and Technology, 18(8), 3787-3792. DOI: 10.52711/0974-360X.2025.00545

. Saputri, L. O., Permatasari, L., Harahap, H. S., Rosyidi, R. M., Rivarti, A. W., Prihatina, L. M., Rahayu, Z., & Azariani, W. (2025). Potential of rutin from Rhizophora mucronata leaves as a Inhibitor of Kelch-like ECH-associated protein 1/Nuclear factor erythroid 2 related factor 2 Keap1/Nrf2): An in silico study for Alzheimer's therapy. Journal of Advanced Pharmaceutical Technology & Research, 16(2), 73. https://doi.org/10.4103/JAPTR.JAPTR_313_24

. Maziya, R., Permatasari, L., Deccati, R. F., Muliasari, H., Rahman, F., Annas, Z. F., & Sammanta, R. V. (2025). Exploration of secondary metabolite profile in the n-hexane fraction of Rhizophora mucronata, Avicennia marina, and Sonneratia alba | Acta Chimica Asiana. https://aca.unram.ac.id/index.php/ACA/article/view/231

. Permatasari, L., sugeng, riyanto, & Rohman, A. (2020). Identification of Phenolic Compound from Active Subfraction Of Baccaurea Racemosa Pulp by High Resolution Mass Spectrometry. International Journal of Pharmaceutical Research, 13(01), 1065–1072. https://doi.org/10.31838/ijpr/2021.13.01.171

. Dewi, L. B. K., Khusuma, A., Muliasari, H., Permatasari, L., & Widodo, H. (2024). The principal component analysis of antioxidant activities of Songgak, atraditional herbal drink in West Nusa Tenggara, Indonesia. Food Research, 8(5), 245–252. https://doi.org/10.26656/fr.2017.8(5).594

. Suryanto, E., & Taroreh, M. R. I. (2020). Ultrasound-Assisted Extraction Antioksidan Serat Pangan Dari Tongkol Jagung (Zea Mays L.). Chemistry Progress, 12(2), Article 2. https://doi.org/10.35799/cp.12.2.2019.27932

. Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji Aktivitas Antimikroba Dari Ekstrak Dan Fraksi Ascidian Herdmania Momus Dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus Aureus, Salmonella Typhimurium Dan Candida Albicans. Pharmacon, 10(1), Article 1. https://doi.org/10.35799/pha.10.2021.32758.

. Reymond, C., Dubuis, A., Le Masle, A., Colas, C., Chahen, L., Destandau, E., & Charon, N. (2020). Characterization of liquid–liquid extraction fractions from lignocellulosic biomass by high performance liquid chromatography hyphenated to tandem high-resolution mass spectrometry. Journal of Chromatography A, 1610, 460569. https://doi.org/10.1016/j.chroma.2019.460569.

. Triadisti, N., & Zamzani, I. (2023). Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves. Borneo Journal of Pharmacy, 6(1), Article 1. https://doi.org/10.33084/bjop.v6i1.1830.

. Platzer, M., Kiese, S., Herfellner, T., Schweiggert-Weisz, U., Miesbauer, O., & Eisner, P. (2021). Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays. Molecules, 26(5), 1244. https://doi.org/10.3390/molecules26051244.

. Pujiawati, Y., Khotijah, L., & Wiryawan, I. (2023). Screening of antioxidant activities and their bioavailability of tropical plants. IOP Conference Series: Earth and Environmental Science, 1182(1), 012083. https://doi.org/10.1088/1755-1315/1182/1/012083.

. Nie, F., Liu, L., Cui, J., Zhao, Y., Zhang, D., Zhou, D., Wu, J., Li, B., Wang, T., Li, M., & Yan, M. (2023). Oligomeric Proanthocyanidins: An Updated Review of Their Natural Sources, Synthesis, and Potentials. Antioxidants, 12(5), Article 5. https://doi.org/10.3390/antiox1205100.

. Puspitasari, A. D., & Wulandari, R. L. (2017). Antioxidant activity, determination of total phenolic and flavonoid content of Muntingia calabura L. Extracts. Pharmaciana, 7(2), Article 2. https://doi.org/10.12928/pharmaciana.v7i2.7104.

. Zeb, A. (2020). Concept, mechanism, and applications of phenolic antioxidants in foods. Journal of Food Biochemistry, 44(9), e13394. https://doi.org/10.1111/jfbc.13394

. Contreras Puentes, N., & Alviz, A. (2020). Hypoglycaemic Property of Yacon (Smallanthus sonchifolius (Poepp. and Hendl.) H. Robinson): A Review. Pharmacognosy Reviews, 14, 37–44.

. Hassanpour, S. H., & Doroudi, A. (2023). Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna Journal of Phytomedicine, 13(4), 354–376. https://doi.org/10.22038/AJP.2023.21774.

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Gravity column chromatography of n-hexane fraction of Rhizophora mucronata leaves from West Nusa Tenggara and their antioxidant activity

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