Determination of Total Flavonoid Content from Extract and Fractions of Mangrove Leaves (Rhizopora mucronata)

Rahula Vijja  Sammanta; Lina Permatasari; Handa Muliasari; Neneng Rachmalia Izzatul Mukhlishah; Rizqa Fersiyana Deccati

doi:10.29303/aca.v7i2.199

$Determination of total flavonoid content from extract and fractions of mangrove (Rhizopora mucronata) leaves$

Determination of Total Flavonoid Content from Extract and Fractions of Mangrove Leaves (Rhizopora mucronata)

Penulis

Rahula Vijja Sammanta , Lina Permatasari , Handa Muliasari , Neneng Rachmalia Izzatul Mukhlishah , Rizqa Fersiyana Deccati

DOI:

10.29303/aca.v7i2.199

Diterbitkan:

2024-10-31

Terbitan:

Vol 7 No 2 (2024)

Kata Kunci:

Rhizopora mucronata leaves, total flavonoid content, spectrophotometry UV/Vis, Thin Layer Chromatography, Mangrove

Articles

Cara Mengutip

Sammanta, R. V., Permatasari, L., Muliasari, H., Rachmalia Izzatul Mukhlishah, N., & Fersiyana Deccati, R. (2024). Determination of Total Flavonoid Content from Extract and Fractions of Mangrove Leaves (Rhizopora mucronata). Acta Chimica Asiana, 7(2), 502–508. https://doi.org/10.29303/aca.v7i2.199

Unduh Sitasi

Unduhan

Data unduhan belum tersedia.

Metrik

Metrik sedang dimuat ...

Abstrak

Indonesia is an archipelagic country with a very wide coastal area. Lombok Island has a coastal area with quite extensive mangrove forest areas. Rhizopora mucronata was the most dominant species found in the region. The Rhizopora mucronata species was a plant from the Rhizoporaceae family reported to have antibacterial, antifungal, and antioxidant activity. These biological activities were caused by the presence of secondary metabolite compounds in R. mucronata, one of which is flavonoids. Research regarding the flavonoid content of R. mucronata leaves has not been explored. Therefore, the study aimed to determine the total flavonoid content in extracts and fractions of R. mucronata leaves using the spectrophotometric UV-Vis method. R. mucronata mangrove leaves simplicia were extracted using the sonication method using 96% ethanol solvent, then fractionated using the liquid-liquid extraction method with n-hexane, ethyl acetate, and water. The percentage yield of 96% ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction respectively were 42.33%; 43.147%; 40.49%; 9.74%; and 32.49%. The TLC test showed the presence of flavonoid compounds detected in the extract and fractions which were marked with blue spots. The total flavonoid contents in the 96% ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction were respectively (12,980; 14,160; 23,880; and 25,350) mg Quercetin equivalent/gram sample (mg QE/ g). The total flavonoid content of the extract and fractions of R. mucronata leaves were analyzed using the One-way ANOVA test method. The total flavonoid content in the n-hexane fraction and ethyl acetate fraction was significantly higher than the 96% ethanol extract and water fraction. The total flavonoid content of R. mucronata leaves was relatively high, which contributes to several of its biological activities.

Referensi

Egra, S., Mardhiana, Rofin, M., Adiwena, M., Jannah, N., Kuspradini, H., & Mitsunaga, T. (2019). Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu. Agrovigor: Jurnal Agroekoteknologi, 12(1), 26. https://doi.org/10.21107/agrovigor.v12i1.5143

Andini, A. S., & Rahayu, S. M. (2019). Kesesuaian Vegetasi dalam Mitigasi Bencana Tsunami di Teluk Sepi, Lombok Barat. Media Bina Ilmiah, 14(3), 2095. https://doi.org/10.33758/mbi.v14i3.310

Kadir, M. A., Wibowo, E. S., Abubakar, S., & Akbar, N. (2019). Manfaat Mangrove Bagi Peruntukan Sediaan Farmasitika di Desa Mamuya Kecamatan Galela Timur Kabupaten Halmahera Timur (Tinjauan Etnofarmakologis). Jurnal Enggano, 4(1), 12–25. https://doi.org/10.31186/jenggano.4.1.12-25

Batool, N., & Ilyas, N. (2014). Asiatic Mangrove (Rhizophora mucronata) An overview. European Academic Research, 2(3), 3351.

Kusumowati, I. T. D. (2012). Korelasi Kandungan Fenolik dan Aktivitas Antiradikal Ekstrak Etanol Daun Empat Tanaman Obat Indonesia (Piper bettle, Sauropus androgynus, Averrhoa bilimbi, dan Guazuma ulmifolia). Pharmacon: Jurnal Farmasi Indonesia, 13(1), 1–5. https://doi.org/10.23917/pharmacon.v13i1.19

Manik, D. F., Hertiani, T., & Anshory, H. (2014). Analisis Korelasi Antara Kadar Flavonoid dengan Aktivitas Antibakteri Ekstrak Etanol dan Fraksi-fraksi Daun Kersen (Muntingia calabura L.) terhadap Staphylococcus aureus. Khazanah, 6(2), 1–11. https://doi.org/10.20885/khazanah.vol6.iss2.art1

Gurudeeban, S., Kaliamurthi, S., Sheik, H. S., & Thiruganasambandam, R. (2014). Molecular docking, isolation and biological evaluation of Rhizophora mucronata flavonoids as anti-nociceptive agents. Biomedicine & Preventive Nutrition, 4(4), 555–560. https://doi.org/10.1016/j.bionut.2014.08.002

Thirunavukkarasu, P., Asha, S., Ramanathan, T., Kannan, D., & Sudhakar, N. (2017). Phytochemical Analysis of Mangrove Derived Crude Plant Extract- Rhizophora Mucronata. Journal of Global Trends in Pharmaceutical Sciences, 8(3), 3813–3820.

Annas, Z.F., Muliasari H, Deccati, R.F., Permatasari, L. & Mukhlishah, N. R. I. (2023). Penentuan kadar flavonoid total ekstrak dan fraksi-fraksi daun mangrove (Avicennia marina) Determination of total flavonoid content of extract and fractions of mangrove leaves (Avicennia marina). Journal Aggrotek UMMAT, 10(3), 271–282.

Mahran, I. T., Suhartini, S., & Sucipto, S. (2019). Optimasi Ekstraksi Ultrasonik Daun Kecubung Sebagai Agen Anti Bakteri Jerawat Propionibacterium Acnes (Kajian: Raio Pelarut Terhadap Bahan dan Lama Waktu Sonikasi). Jurnal Keteknikan Pertanian Tropis dan Biosistem, 7(2), 137–147

Chen, A., Xiang, W., Liu, D., Liu, C., & Yang, L. (2016). Determination of Total Flavonoids and Its Antioxidant Ability in Houttuynia cordata. Journal of Materials Science and Chemical Engineering, 04(02), 131–136. https://doi.org/10.4236/msce.2016.42014

Zhang, Q.-W., Lin, L.-G., & Ye, W.-C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine, 13(1), 20. https://doi.org/10.1186/s13020-018-0177-x

Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. (2021). Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.). Jurnal Pijar Mipa, 16(3), 397–405. https://doi.org/10.29303/jpm.v16i3.2308

Li, C., Ge, Y., Wan, D., Hu, J., Ying, C., & Wang, L. (2011). Optimization of Extraction Condition and Quantification of Total Flavonoids in Elaeagni Folium. Pharmacognosy Journal, 3(26), 8–12. https://doi.org/10.5530/pj.2011.26.2

Desiani, E., Mardiana, T. Y., Madusari, B. D., & Hidayat, F. N. (2022). Uji Aktivitas Analgesik Ekstrak Daun Mangrove (Rhizophora mucronata) pada Mencit yang diinduksi Asam Asetat dengan Metode Writhing Reflex. Cendekia Journal of Pharmacy, 2(2), 307–317.

Handayani, D., Mun’im, A., & Ranti, A. S. (2014). Optimasi Ekstraksi Ampas Teh Hijau (Camellia sinensis) Menggunakan Metode Microwave Assisted Extraction Untuk Menghasilkan Ekstrak Teh Hijau. Trad. Med. J, 19(1), 7.

Widarta, I. W. R., & Arnata, I. W. (2017). Ekstraksi Komponen Bioaktif Daun Alpukat dengan Bantuan Ultrasonik pada Berbagai Jenis dan Konsentrasi Pelarut. Agritech, 37(2), 148. https://doi.org/10.22146/agritech.10397

Anjaswati, D., Pratimasari, D., & Nirwana, A. P. (2021). Perbandingan Rendemen Ekstrak Etanol, Fraksi n- Heksana, Etil Asetat, dan Air Daun Bit (Beta vulgaris L.) Menggunakan Fraksinasi Bertingkat. Jurnal Farmasi (Journal of Pharmacy), 2(337), 5.

Mustarichie, R. (2017). The antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate and n-hexane from mistletoe tea (Scurrula atropurpureabl. Dans). Asian Journal of Pharmaceutical and Clinical Research, 10, 343. https://doi.org/10.22159/ajpcr.2017.v10i2.15724

Permatasari, L., Riyanto, S., & Rohman, A. (2019). Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. pulp: A potential naturalantioxidant. Food Research Journal, 3(6), 713–719. https://doi.org/10.26656/fr.2017.3(6).165

Fadhlillah, F. M., Oktaviani, W., & Mariani, R. (2019). Antioxidant activity of Ethanol Extract, n-Hexane fraction, Ethyl Acetate fraction and Water fraction of Garut Orange Leaves (Citrus reticulata Blanco). Journal of Physics: Conference Series, 1402(5), 055083. https://doi.org/10.1088/1742-6596/1402/5/055083

Ikalinus, R., Widyastuti, S. K., & Setiasih, N. L. E. (2015). Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa oleifera). Indonesia Medicus Veterinus, 4(1), 9.

Iskandar, D. (2020). Aplikasi Uji Skrining Fitokimia Terhadap Daun Uncaria tomentosa Sebagai Bahan Utama Dalam Pembuatan Teh. Jurnal Teknologi Technoscientia, 12(2), 153–158. https://doi.org/10.34151/technoscientia.v12i2.2659

Mariana, L., & Andayani, Y. (2013). Analisis Senyawa Flavonoid Hasil Fraksinasi Ekstrak Diklorometana Daun Keluwih. Chem Prog, 6(2), 52–53.

Haeria, Hermawati, & Pine, A. T. U. (2016). Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Bidara (Ziziphus spina-christi L.). Journal of Pharmaceutical and Medicinal Sciences, 1(2), 57–61.

Sopiah, B., Muliasari, H., & Yuanita, E. (2019). Skrining Fitokimia dan Potensi Aktivitas Antioksidan Ekstrak Etanol Daun Kastuba (Euphorbia pulcherrima Willd.). Jurnal Ilmu Kefarmasian Indonesia, 17(1), 27–33.

Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 2013(1), 1–16. https://doi.org/10.1155/2013/162750

Razoki, Butar-Butar, R. G. S., Neswita, E., Sembiring, N. B., Novriani, E., Simanjuntak, N. J. P., & Pakpahan, E. H. (2023). Uji skrining fitokimia dan pengukuran kadar total flavonoid pada ekstrak paku (Nephrolepis biserrata) dengan fraksi n-heksana, etil asetat, dan air. Journal of Pharmaceutical and Sciences, 6(3), Article 3. https://doi.org/10.36490/journal-jps.com.v6i3.185

Bajalan, I., Zand, M., Goodarzi, M., & Darabi, M. (2017). Antioxidant activity and total phenolic and flavonoid content of the extract and chemical composition of the essential oil of Eremostachys laciniata collected from Zagros. Asian Pacific Journal of Tropical Biomedicine, 7(2), 144–146. https://doi.org/10.1016/j.apjtb.2016.11.022

Xie, L., Deng, Z., Zhang, J., Dong, H., Wang, W., Xing, B., & Liu, X. (2022). Comparison of Flavonoid O-Glycoside, C-Glycoside and Their Aglycones on Antioxidant Capacity and Metabolism during In Vitro Digestion and In Vivo. Foods, 11(6), 882. https://doi.org/10.3390/foods11060882

Lisensi

Artikel ini berlisensiCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Authors who publish with ACA: Acta Chimica Asiana agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in ACA: Acta Chimica Asiana.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Determination of Total Flavonoid Content from Extract and Fractions of Mangrove Leaves (Rhizopora mucronata)

Penulis

DOI:

Diterbitkan:

Terbitan:

Kata Kunci:

Articles

Cara Mengutip

Unduhan

Metrik

Abstrak

Referensi

Lisensi

Authors who publish with ACA: Acta Chimica Asiana agree to the following terms:

Artikel paling banyak dibaca berdasarkan penulis yang sama

menu

Manuscript Template

Bahasa

Visitor

Terbitan Terkini

Journal Publisher

Contact Us

Information

Share & Follow